Coding
Part:BBa_K2936003:Design
Designed by: Yu Weng Group: iGEM19_ZJUT-China (2019-10-08)
S- glutathione hydrolase(Regulated by flhA gene)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 130
Illegal PstI site found at 610 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 130
Illegal PstI site found at 610
Illegal NotI site found at 971 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 130
Illegal BglII site found at 491 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 130
Illegal PstI site found at 610 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 130
Illegal PstI site found at 610
Illegal NgoMIV site found at 79
Illegal NgoMIV site found at 275
Illegal NgoMIV site found at 628
Illegal NgoMIV site found at 888 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
When combined with fghA gene sequence, an RBS sequence needs to be inserted between the two ends of genes to improve the translation speed.
Source
From Parazococci denitrification.